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c j k t c 1 d ow nloaded from  (Addgene inc)


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    Structured Review

    Addgene inc c j k t c 1 d ow nloaded from
    C J K T C 1 D Ow Nloaded From, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+j+k+t+c+1+d+ow+nloaded+from/pH7SB-112+(Plasmid+%2385260)/pm41841497-66-84-130
    Average 94 stars, based on 1 article reviews
    c j k t c 1 d ow nloaded from - by Bioz Stars, 2026-11
    94/100 stars

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    Polymerase Chain Reaction:

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage.
    Article Snippet: Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen 69504) and employed for PCR using Illumina adapters to identify the gRNA representation in each sample. .. Then, 10 μg of gDNA was used in each PCR reaction along with 20 μl 5 × HiFi Reaction Buffer, 4 μl of P5 primer, 4 μl of P7 primer, 3 μl of R T p o e c f fi u p P s B e b i g M t F p p F N p v w s o a m u a p s F p c a a w P F e 0 r 1 b 3 i s c ( j k t c 1 D ow nloaded from https://academ ic.oup.com /nar/article/54/5/gkag226/8526029 by guest on 20 M arch 2026 adiant HiFi Ultra Polymerase (Stellar Scientific), and water. he P5 and P7 primers were determined using the user guide rovided with the CRISPR libraries ( https://media.addgene. rg/ cms/ filer _ public/ 61/ 16/ 611619f4- 0926- 4a07- b5c7286a8ecf7f5/broadgpp- sequencing- protocol.pdf). .. Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen 69504) and employed for PCR using Illumina adapters to identify the gRNA representation in each sample.

    CRISPR:

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage.
    Article Snippet: Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen 69504) and employed for PCR using Illumina adapters to identify the gRNA representation in each sample. .. Then, 10 μg of gDNA was used in each PCR reaction along with 20 μl 5 × HiFi Reaction Buffer, 4 μl of P5 primer, 4 μl of P7 primer, 3 μl of R T p o e c f fi u p P s B e b i g M t F p p F N p v w s o a m u a p s F p c a a w P F e 0 r 1 b 3 i s c ( j k t c 1 D ow nloaded from https://academ ic.oup.com /nar/article/54/5/gkag226/8526029 by guest on 20 M arch 2026 adiant HiFi Ultra Polymerase (Stellar Scientific), and water. he P5 and P7 primers were determined using the user guide rovided with the CRISPR libraries ( https://media.addgene. rg/ cms/ filer _ public/ 61/ 16/ 611619f4- 0926- 4a07- b5c7286a8ecf7f5/broadgpp- sequencing- protocol.pdf). .. Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen 69504) and employed for PCR using Illumina adapters to identify the gRNA representation in each sample.

    Sequencing:

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage.
    Article Snippet: Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen 69504) and employed for PCR using Illumina adapters to identify the gRNA representation in each sample. .. Then, 10 μg of gDNA was used in each PCR reaction along with 20 μl 5 × HiFi Reaction Buffer, 4 μl of P5 primer, 4 μl of P7 primer, 3 μl of R T p o e c f fi u p P s B e b i g M t F p p F N p v w s o a m u a p s F p c a a w P F e 0 r 1 b 3 i s c ( j k t c 1 D ow nloaded from https://academ ic.oup.com /nar/article/54/5/gkag226/8526029 by guest on 20 M arch 2026 adiant HiFi Ultra Polymerase (Stellar Scientific), and water. he P5 and P7 primers were determined using the user guide rovided with the CRISPR libraries ( https://media.addgene. rg/ cms/ filer _ public/ 61/ 16/ 611619f4- 0926- 4a07- b5c7286a8ecf7f5/broadgpp- sequencing- protocol.pdf). .. Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen 69504) and employed for PCR using Illumina adapters to identify the gRNA representation in each sample.



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    94
    Addgene inc c j k t c 1 d ow nloaded from
    C J K T C 1 D Ow Nloaded From, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+j+k+t+c+1+d+ow+nloaded+from/pH7SB-112+(Plasmid+%2385260)/pm41841497-66-84-130
    Average 94 stars, based on 1 article reviews
    c j k t c 1 d ow nloaded from - by Bioz Stars, 2026-11
    94/100 stars
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